Intracellular membrane trafficking in the nervous and neuroendocrine systems
Team leaders : Stéphane Gasman & Nicolas Vitale
Functioning of the nervous and neuroendocrine systems is based on the release of informative molecules thanks to various tightly regulated membrane trafficking processes (exocytosis, endocytosis, vesicular transport). Our research aims at deciphering the molecular mechanisms regulating these trafficking events and at unravelling their dysfunction associated with important pathologies (mental retardation, neurodegenerative diseases, neuroendocrine cancers, obesity…). Using neurons, neuroendocrine cells and ependymal cells, we are particularly studying the mechanisms by which different lipids and proteins control the life cycle of vesicles, starting from biogenesis to membrane fusion through their intracellular transport.
Team projects
- Compensatory endocytosis and vesicle recycling
Stéphane Ory - Formation of exocytotic sites
Sylvette Chasserot-Golaz - Lipids and membrane fusion
Nicolas Vitale & Emeline Tanguy - Secretion and neuroendocrine tumors
Stéphane Gasman - Transport of metabolic hormones into the brain
Stéphane Ory & Stéphane Gasman - V-ATPase and vesicular maturation
Nicolas Vitale
Members
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Marie-France Bader (Senior researcher) |
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Sylvette Chasserot-Golaz (Researcher) |
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Stéphane Gasman (Senior researcher) |
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Olga Gubar (Invited Researcher, University of Kiev, Ukraine) |
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Stéphane Ory (Researcher) |
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Emeline Tanguy (Associate Professor) |
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Petra Tóth (Associate professor) |
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Nicolas Vitale (Senior researcher) |
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Alice Basile (Ph.D. Student) |
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Bérengère Joffre (Ph.D. Student) |
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Michael Okafor (Ph.D. Student) |
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Alexander Wolf (Ph.D. Student) |
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Claudine Boissier (Assistant Technician) |
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Anne-Marie Haeberlé (Engineer) |
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Jason Ecard (Research Engineer) |
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Sebahat Ozkan (Assistant Engineer) |
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Laura Streit (Research Engineer) |
Selected publications
Houy S, Streit L, Drissa I, Rame M, Decraene C, Moog S, Brunaud L, Lanoix J, Chelbi R, Bihain F, Lacomme S, Lomazzi S, Campoli P, Vix M, Mutter D, Paramithiotis E, Dubessy C, Vitale N, Ory S, Gasman S. (2022) Dysfunction of calcium-regulated exocytosis at a single-cell level causes catecholamine hypersecretion in patients with pheochromocytoma. Cancer Letters 543: 215765.
Duquenne M, Folgueira C, Bourrouh C, Millet M, Silva A, Clasadonte J, Imbernon M, Fernandois D, Martinez-Corral I, Kusumakshi S, Caron E, Rasika S, Deliglia E, Jouy N, Oishi A, Mazzone M, Trinquet E, Tavernier J, Kim YB, Ory S, Jockers R, Schwaninger M, Boehm U, Nogueiras R, Annicotte JS, Gasman S, Dam J & Prévot V (2021) Leptin enters the brain through a LepR:EGFR shuttle in tanycytes to regulate peripheral lipogenesis and pancreatic beta-cell function. Nature Metabolism Aug;3(8):1071-1090.
Tanguy E, Coste de Bagneaux P, Kassas N, Ammar MR, Wang Q, Haeberle AM, Raherindratsara J, Fouillen L, Renard PY, Montero-Hadjadje M, Chasserot-Golaz S, Ory S, Gasman S, Bader MF, Vitale N (2020) Mono- and Poly-unsaturated Phosphatidic Acid Regulate Distinct Steps of Regulated Exocytosis in Neuroendocrine Cells. Cell Reports 32:108026.
Gubar O, Croise P, Kropyvko S, Gryaznova T, Toth P, Blangy A, Vitale N, Rynditch A, Gasman S, Ory S (2020) The atypical Rho GTPase RhoU interacts with intersectin-2 to regulate endosomal recycling pathways. Journal of Cell Science Aug 27;133(16):jcs234104.
Gabel M, Delavoie F, Royer C, Tahouly T, Gasman S, Bader MF, Vitale N, Chasserot-Golaz S (2019) Phosphorylation cycling of Annexin A2 Tyr23 is critical for calcium-regulated exocytosis in neuroendocrine cells. Biochimica Biophysica Acta (Mol Cell Res) 1866:1207-1217.
Chopard C, Tong PBV, Toth P, Schatz M, Yezid H, Debaisieux S, Mettling C, Gross A, Pugniere M, Tu A, Strub JM, Mesnard JM, Vitale N, Beaumelle B (2018) Cyclophilin A enables specific HIV-1 Tat palmitoylation and accumulation in uninfected cells. Nature Communications 9:2251.
