Principle and Purpose

Quantitative PCR (real-time PCR, QPCR) uses the basic principle of classical PCR (cyclic amplification of a DNA fragment based on an enzymological reaction) with the difference that an amplification measured not in final but throughout of the reaction, and therefore in real time.

At each amplification cycle, the amount of DNA is measured using a fluorescent marker such as “syber green” whose emission is directly proportional to the amount of amplicons produced. This makes it possible to obtain a kinetics of the reaction and thus the quantification of the DNA whereas the conventional PCR only gives the final measurement. Thus this technique makes it possible to compare the differences in expression of the genes as functions of the experimental parameters defined by the researcher.

Equipment

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