The French National Centre for Scientific Research


Molecular biology and bioinformatics

Objective

The purpose of the Molecular Biology and Bioinformatics platform is to provide technical support and propose solutions for all related research activities performed at INCI.


 

Technics

  • Extraction, purification of nucleic acids
  • PCR amplification
  • Cloning (classical plasmid and for viral expression)
  • Quantification and quality control of nucleic acids and PCR products
  • Pipeting robot QIAgility
  • 384-well quantitative PCR
  • Molecular biology training and consulting (pipeting robot use, cloning)
  • Data analysis: sequencing (RNAseq, WGBS, EMseq, etc) and micrarrays (Infinium MethylationEPIC)
  • Data analysis training and consulting (R, Linux)

Equipment

  • Gel electrophoresis
  • Thermo Scientific Sorvall Lynx 4000 centrifuge
  • Eppendorf 5424R centrifuge
  • MasterCycler gradients Eppendorf Thermal Cycler
  • Applied Biosystems Simply Amp Thermal Cycler 96w
  • Eppendorf BioPhotometer
  • Nanodrop 2000c Thermo Scientific
  • ThermoFischer Qubit fluorometer
  • Gel Doc EZimager Bio-Rad
  • Agilent Bioanalyzer capillary electrophoresis

 

Members & Contacts

The platform is located on the 2nd floor of the Institute, room 2006.

Naïma El-Kholti
(Assistante ingénieur)
E-mail
 
Sebahat Ozkan
(Assistante ingénieur)
E-mail
 
Charles Decreane
(Ingénieur de Recherche)
E-mail
 
Nicolas Vitale
(Directeur de recherche)
E-mail
 
Pierre-Eric Lutz
(Chargé de recherche)
E-mail
 

 


 
The French National Centre for Scientific Research