Objective

The purpose of the Molecular Biology and Bioinformatics platform is to provide technical support and propose solutions for all related research activities performed at INCI.

Technics

• Extraction, purification of nucleic acids
• PCR amplification
• Cloning (classical plasmid and for viral expression)
• Quantification and quality control of nucleic acids and PCR products
• Pipeting robot QIAgility
• 384-well quantitative PCR
• Molecular biology training and consulting (pipeting robot use, cloning)
• Data analysis: sequencing (RNAseq, WGBS, EMseq, etc) and micrarrays (Infinium MethylationEPIC)
• Data analysis training and consulting (R, Linux)

Equipment

• Gel electrophoresis
• Thermo Scientific Sorvall Lynx 4000 centrifuge
• Eppendorf 5424R centrifuge
• MasterCycler gradients Eppendorf Thermal Cycler
• Applied Biosystems Simply Amp Thermal Cycler 96w
• Eppendorf BioPhotometer
• Nanodrop 2000c Thermo Scientific
• ThermoFischer Qubit fluorometer
• Gel Doc EZimager Bio-Rad
• Agilent Bioanalyzer capillary electrophoresis

Members & Contacts

The platform is located on the 2nd floor of the Institute, room 2006.

Naïma El-Kholti (Assistante ingénieur) E-mail

Sebahat Ozkan (Assistante ingénieur) E-mail

Charles Decreane (Ingénieur de Recherche) E-mail

Nicolas Vitale (Directeur de recherche) E-mail

Pierre-Eric Lutz (Chargé de recherche) E-mail