Molecular biology and bioinformatics
Objective
The purpose of the Molecular Biology and Bioinformatics platform is to provide technical support and propose solutions for all related research activities performed at INCI.
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Technics
- Extraction, purification of nucleic acids
- PCR amplification
- Cloning (classical plasmid and for viral expression)
- Quantification and quality control of nucleic acids and PCR products
- Pipeting robot QIAgility
- 384-well quantitative PCR
- Molecular biology training and consulting (pipeting robot use, cloning)
- Data analysis: sequencing (RNAseq, WGBS, EMseq, etc) and micrarrays (Infinium MethylationEPIC)
- Data analysis training and consulting (R, Linux)
Equipment
- Gel electrophoresis
- Thermo Scientific Sorvall Lynx 4000 centrifuge
- Eppendorf 5424R centrifuge
- MasterCycler gradients Eppendorf Thermal Cycler
- Applied Biosystems Simply Amp Thermal Cycler 96w
- Eppendorf BioPhotometer
- Nanodrop 2000c Thermo Scientific
- ThermoFischer Qubit fluorometer
- Gel Doc EZimager Bio-Rad
- Agilent Bioanalyzer capillary electrophoresis
Members & Contacts
The platform is located on the 2nd floor of the Institute, room 2006.
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Naïma El-Kholti (Assistante ingénieur) |
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Sebahat Ozkan (Assistante ingénieur) |
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Charles Decreane (Ingénieur de Recherche) |
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Nicolas Vitale (Directeur de recherche) |
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Pierre-Eric Lutz (Chargé de recherche) |