The French National Centre for Scientific Research

Unraveling modular information processing in the cerebellar cortex in normal and pathological conditions

Project leader : Philippe ISOPE

The cerebellum plays a major role in the control and learning of skilled movements, and in the timing of our actions. Despite its macroscopic isotropy, the cerebellar cortex is compartmentalized into biochemically- and functionally-defined modules. Indeed, Purkinje cells belong to multiple classes that form an array of transverse zones and parasagittal stripes, which can be identified by histochemical markers called Zebrins. Functional studies have demonstrated that task-related modules can be identified and selectively modified.

Our main questions are:
How is spatial and temporal information processed in cerebellar modules?
How is specific information communicated to other brain areas in normal and pathological conditions?

To address these issues, we combine in vitro and in vivo electrophysiological recordings with two photon imaging and optogenetic techniques in rodent cerebellum.

Cerebellar slice of a glyt2 mice (in green and blue, Golgi cells) stained by a Zebrin II antibody (in red, Aldolase C positive Purkinje cells, antibody from R Hawkes, Calgary)


Philippe Isope
Fred Doussau
Frédéric Doussau
Bernard Poulain
Bernard Poulain

Aline Huber
Ludo Spaeth
Ludovic Spaeth
Federica Lareno-Faccini
Federica Lareno-Faccini
PhD student

Théo Gagneux
PhD student


Fekrije Selimi, Collège de France, Paris, France
Daniela Popa et Clément Léna, IBENS, Paris, France
Cathie Ventalon, IBENS, Paris, France
David Digregorio, Institut Pasteur, Paris, France
Jean Livet, Institut de la Vision, Paris, France
Emmanuel Beaurepaire, Ecole Polytechnique, Paris-Saclay, France
Arvind Kumar, KTH, Stockholm, Sweden

Selected publications

Dorgans K, Demais V, Bailly Y, Poulain B, Isope P, Doussau F. Short-term plasticity at cerebellar granule cell to molecular layer interneuron synapses expands information processing.Elife. 2019 May 13;8. pii: e41586.

Doussau F, Schmidt H, Dorgans K, Valera AM, Poulain B, Isope P. Frequency-dependent mobilization of heterogeneous pools of synaptic vesicles shapes presynaptic plasticity. Elife. 2017 Oct 9;6. pii: e28935.

Stereotyped spatial patterns of functional synaptic connectivity in the cerebellar cortex. Valera AM, Binda F, Pawlowski SA, Dupont JL, Casella JF, Rothstein JD, Poulain B, Isope P.Elife. 2016 Mar 16;5. pii: e09862.

Proville RD, Spolidoro M, Guyon N, Dugué GP, Selimi F, Isope P, Popa D, Léna C. Cerebellum involvement in cortical sensorimotor circuits for the control of voluntary movements. Nat Neurosci. 2014 Sep;17(9):1233-9.

Chaumont J, Guyon N, Valera AM, Dugué GP, Popa D, Marcaggi P, Gautheron V, Reibel-Foisset S, Dieudonné S, Stephan A, Barrot M, Cassel JC, Dupont JL, Doussau F, Poulain B, Selimi F, Léna C, Isope P. Clusters of cerebellar Purkinje cells control their afferent climbing fiber discharge. PNAS. 2013 Oct 1;110(40):16223-8.


The French National Centre for Scientific Research