The French National Centre for Scientific Research


V-ATPase and vesicular maturation

Project leader : Nicolas Vitale


The proton gradient generated by the V-ATPase is directly used by specific transporters to load neurotransmetters and chemical messenger molecules into secretory vesicles. However an additional more direct role for V-ATPases has been proposed in membrane fusion, although the precise molecular mechanisms involves have remained a mistery. The objective of this project is to investigate whether V-ATPase, via V0/V1 sub-domaines association/dissociation, could contribute to determine the maturity status of secretory granules and thus allow specific exocytosis of fully loaded vesicles. The last part of the project is devoted to the characterization of the molecular pathway allowing V0 to regulate fusion port formation and expansion, with a focus on the synthesis of fusogenic lipids at the exocytotic sites, and especially phosphatidic acid.


Members

Nicolas Vitale Nicolas Vitale
(Senior researcher)
 
Claudine Boissier Claudine Boissier
 
Anne-Marie Haeberlé Anne-Marie Haeberlé
 
Tamou Thahouly Tamou Thahouly
 

Collaborators

– Yves Mely, Laboratory of bio-imaging and pathologies, CNRS, Illkirch-Graffenstaden, France
– Oussama El Far, Dpt. of ion channels and synapse neurobiology, INSERM, Marseille, France



Selected publications

Tanguy E, Carmon O, Wang Q, Jeandel L, Chasserot-Golaz S, Montero-Hadjadje M, Vitale N (2016) Lipids implicated in the journey of a secretory granule: from biogenesis to fusion. J Neurochem. 137:904-12.

Morel N, Vitale N. (2014) V-ATPase is a pH sensor controlling vesicular membrane fusion. Med Sci (Paris) 30: 631-3.

Poëa-Guyon S, Ammar MR, Erard M, Amar M, Moreau AW, Fossier P, Gleize V, Vitale N, Morel N . (2013) The V-ATPase membrane domain is a sensor of granular pH that controls the exocytotic machinery. J Cell Biol. 137:203 : 283-98.

 


 
The French National Centre for Scientific Research