The French National Centre for Scientific Research


Lipids and membrane fusion

Project leader : Nicolas Vitale



Exocytosis is a multistep process allowing release of informative molecules stores in dedicated secretory organelles for chemical cell-to-cell communication. Even if this process involves specific membrane compartments, the role of lipids the main constituents of membrane remains poorly understood. The goal of this project is to understand the role of the different forms of phosphatidic acid (PA) along the different steps of exocytosis. To address these questions we will generate a novel molecular toolbox dedicated to study the pleiotropic function of PA, largely based on optogenetic approaches. We will also identify the targets of these different forms of PA to define the molecular mechanisms by which PA contribute to exocytosis. The last part of the project is dedicated to study the pathophysiological consequences of alterations in PA synthesis in neurosecreting cells with a specific focus on intellectual disabilities and neurodegenerative diseases.


Members

Nicolas Vitale Nicolas Vitale
(Senior researcher)
 
Marie-France Bader Marie-France Bader
(Senior researcher)
 
Sylvette Chasserot-Golaz Sylvette Chasserot-Golaz
(Researcher)
 
Emeline Tanguy Emeline Tanguy
(Postdoctoral fellow)
 
Michael Okafor Michael Okafor
(Ph.D student)
 
Alexander Wolf Alexander Wolf
(Ph.D student)
 
Claudine Boissier Claudine Boissier
 
Anne-Marie Haeberlé Anne-Marie Haeberlé
 
Tamou Thahouly Tamou Thahouly
 

Collaborators

– Maité Montero, DC2N Laboratory, Inserm, IRIB, Rouen University, Mont-Saint-Aignan, France
– Pierre-Yves Renard, COBRA Laboratory, CNRS, IRIB, Rouen University, Mont-Saint-Aignan, France
– Hervé Moine, IGBMC, Strasbourg



Selected publications

Tanguy E, Coste de Bagneaux P, Kassas N, Ammar MR, Wang Q, Haeberle AM, Raherindratsara J, Fouillen L, Renard PY, Montero-Hadjadje M, Chasserot-Golaz S, Ory S, Gasman S, Bader MF, Vitale N (2020) Mono- and Poly-unsaturated Phosphatidic Acid Regulate Distinct Steps of Regulated Exocytosis in Neuroendocrine Cells. Cell Rep 32:108026.

Carmon O, Laguerre F, Riachy L, Delestre-Delacour C, Wang Q, Tanguy E, Jeandel L, Cartier D, Thahouly T, Haeberlé AM, Fouillen L, Rezazgui O, Schapman D, Haefelé A, Goumon Y, Galas L, Renard PY, Alexandre S, Vitale N, Anouar Y, Montero-Hadjadje M. (2020) Chromogranin A preferential interaction with Golgi phosphatidic acid induces membrane deformation and contributes to secretory granule biogenesis. FASEB J. 34:6769-6790.

Tanguy E, Wang Q, Moine H, and Vitale N (2019) Phosphatidic acid: from pleiotropic functions to neuronal pathology. Front Cell Neurosci. 13:2.

Kassas N, Tanguy E, Thahouly T, Fouillen L, Heintz D, Chasserot-Golaz S, Bader MF, Grant NJ, Vitale N (2017) Comparative characterization of phosphatidic acid sensors and their localization during frustrated phagocytosis. J Biol Chem. 292: 4266-4279.

Tabet R, Moutin E, Becker JA, Heintz D, Fouillen L, Flatter E, Krężel W, Alunni V, Koebel P, Dembélé D, Tassone F, Bardoni B, Mandel JL, Vitale N, Muller D, Le Merrer J, Moine H (2016) Fragile X Mental Retardation Protein controls diacylglycerol kinase activity in neurons. Proc. Natl. Acad. Sci. USA 113, E3619-28.

 


 
The French National Centre for Scientific Research