The French National Centre for Scientific Research


Formation of exocytotic sites

Project leader : Sylvette Chasserot-Golaz


The objective of this project is to show that exocytotic sites are lipid platforms constituting assembly sites of the molecular machineries involved in neuroendocrine secretion. For this purpose the composition of these platforms and their formation mechanisms involving Annexin A2 and actin are studied. The electron tomography revealed different actin structures associated with granules anchored to the plasma membrane. We try to determine the role of these different actin structures and their origin. The functional importance of the contacts between the endoplasmic reticulum and the plasma membrane emerges in particular concerning the impact of the organization of the plasma membrane on specific cellular functions such as exocytosis and endocytosis. The last part of the project is devoted to the characterization of membrane contacts of the endoplasmic reticulum at exocytosis sites.


Members

Sylvette Chasserot-Golaz Sylvette Chasserot-Golaz
(Researcher)
 
Claudine Boissier Claudine Boissier
 
Anne-Marie Haeberlé Anne-Marie Haeberlé
 
Tamou Thahouly Tamou Thahouly
 

Collaborators

– Franck Delavoie, Laboratory of eucaryotic molecular biology, CNRS, Paul Sabatier University,Toulouse, France.



Selected publications

Gabel M, Royer C, Thahouly T, Calco V, Gasman S, Bader MF, Vitale N, Chasserot-Golaz S (2020) Annexin A2 Egress during Calcium-Regulated Exocytosis in Neuroendocrine Cells. Cells 9. E2059.

Gabel M, Delavoie F, Royer C, Tahouly T, Gasman S, Bader MF, Vitale N, Chasserot-Golaz S (2019) Phosphorylation cycling of Annexin A2 Tyr23 is critical for calcium-regulated exocytosis in neuroendocrine cells. Biochim Biophys Acta Mol Cell Res 1866:1207-1217.

Gabel M, Chasserot-Golaz S (2016) Annexin A2, an essential partner of the exocytotic process in chromaffin cells. J Neurochem 137:890-896.

Gabel M, Delavoie F, Demais V, Royer C, Bailly Y, Vitale N, Bader MF, Chasserot-Golaz S (2015) Annexin A2-dependent actin bundling promotes secretory granule docking to the plasma membrane and exocytosis. J Cell Biol 210:785-800.

 


 
The French National Centre for Scientific Research