The French National Centre for Scientific Research


Compensatory endocytosis and vesicular recycling


Project leader : Stéphane Ory


The plasma membrane is an asymmetric phospholipid bilayer which is hugely reorganized in neurosecretory cells mostly during calcium-regulated exocytosis. Fast and massive supply of vesicular membranes must be compensated by endocytosis to maintain plasma membrane homeostasis. Molecular mechanisms involved in exocytosis-endocytosis coupling are still not well understood. Our results indicate that the plasma membrane asymmetry, the Phospholipid Scramblase-1 (PLSCR1) and Rho GTPases regulators are instrumental in exocytosis-endocytosis coupling in neurosecretory cells. Using cell imaging approaches and biochemistry, we characterize molecular events that lead to lipids and protein recapture after exocytosis.



Members

Stéphane Ory Stéphane Ory
(Researcher)
 
Marie-France Bader Marie-France Bader
(Senior researcher)
 
Sylvette Chasserot-Golaz Sylvette Chasserot-Golaz
(Researcher)
 
Stéphane Gasman Stéphane Gasman
(Senior researcher)
 
Petra Tóth Petra Tóth
(Associate professor)
 
Margherita Caputo Margherita Caputo
(Ph. D. student)
 
Claudine Boissier Claudine Boissier
 
Anne-Marie Haeberlé Anne-Marie Haeberlé
 
Tamou Thahouly Tamou Thahouly
 
Sebahat Ozkan Sebahat Ozkan
 

Collaborators

– Lydia Danglot, Institute of Psychiatry and Neuroscience , INSERM, Paris.
– Toshihide Kobayashi, laboratory of Biophotonics and Pharmacology, CNRS, Strasbourg.



Selected publications

Gubar O, Croise P, Kropyvko S, Gryaznova T, Toth P, Blangy A, Vitale N, Rynditch A, Gasman S, Ory S (2020) The atypical Rho GTPase RhoU interacts with intersectin-2 to regulate endosomal recycling pathways. J Cell Sci Aug 27;133(16):jcs234104.

Houy S, Estay-Ahumada C, Croisé P, Calco V, Haeberlé AM, Bailly Y, Billuart P, Vitale N, Bader MF, Ory S, Gasman S (2015) Oligophrenin-1 connects exocytotic fusion to compensatory endocytosis in neuroendocrine cells. J. Neurosci. 35(31):11045-55.

Croisé P, Estay-Ahumada C, Gasman S, Ory S (2014) RhoGTPases, phosphoinositides, and actin: A tripartite framework for efficient vesicular trafficking. Small GTPases Jun 10;5.

Ory S, Ceridono M, Momboisse F, Houy S, Chasserot-Golaz S, Heintz D, Calco V, Haeberlé AM, Espinoza FA, Sims PJ, Bailly Y, Bader MF, Gasman S (2013) Phospholipid scramblase-1-induced lipid reorganization regulates compensatory endocytosis in neuroendocrine cells. J. Neurosci. 33:3545-56.

Ceridono M, Ory S, Momboisse F, Chasserot-Golaz S, Houy S, Calco V, Haeberlé AM, Demais V, Bailly Y, Bader MF, Gasman S (2011) Selective recapture of secretory granule components after full collapse exocytosis in neuroendocrine chromaffin cells. Traffic 12(1):72-88.

 


 
The French National Centre for Scientific Research