The French National Centre for Scientific Research

Compensatory endocytosis and vesicular recycling

Project leader : Stéphane Ory

The plasma membrane is an asymmetric phospholipid bilayer which is hugely reorganized in neurosecretory cells mostly during calcium-regulated exocytosis. Fast and massive supply of vesicular membranes must be compensated by endocytosis to maintain plasma membrane homeostasis. Molecular mechanisms involved in exocytosis-endocytosis coupling are still not well understood. Our results indicate that the plasma membrane asymmetry, the Phospholipid Scramblase-1 (PLSCR1) and Rho GTPases regulators are instrumental in exocytosis-endocytosis coupling in neurosecretory cells. Using cell imaging approaches and biochemistry, we characterize molecular events that lead to lipids and protein recapture after exocytosis.


Stéphane Ory Stéphane Ory
Marie-France Bader Marie-France Bader
(Senior researcher)
Sylvette Chasserot-Golaz Sylvette Chasserot-Golaz
Stéphane Gasman Stéphane Gasman
(Senior researcher)
Petra Tóth Petra Tóth
(Associate professor)
Margherita Caputo Margherita Caputo
(Ph. D. student)
Claudine Boissier Claudine Boissier
Anne-Marie Haeberlé Anne-Marie Haeberlé
Tamou Thahouly Tamou Thahouly
Sebahat Ozkan Sebahat Ozkan


– Lydia Danglot, Institute of Psychiatry and Neuroscience , INSERM, Paris.
– Toshihide Kobayashi, laboratory of Biophotonics and Pharmacology, CNRS, Strasbourg.

Selected publications

Gubar O, Croise P, Kropyvko S, Gryaznova T, Toth P, Blangy A, Vitale N, Rynditch A, Gasman S, Ory S (2020) The atypical Rho GTPase RhoU interacts with intersectin-2 to regulate endosomal recycling pathways. J Cell Sci Aug 27;133(16):jcs234104.

Houy S, Estay-Ahumada C, Croisé P, Calco V, Haeberlé AM, Bailly Y, Billuart P, Vitale N, Bader MF, Ory S, Gasman S (2015) Oligophrenin-1 connects exocytotic fusion to compensatory endocytosis in neuroendocrine cells. J. Neurosci. 35(31):11045-55.

Croisé P, Estay-Ahumada C, Gasman S, Ory S (2014) RhoGTPases, phosphoinositides, and actin: A tripartite framework for efficient vesicular trafficking. Small GTPases Jun 10;5.

Ory S, Ceridono M, Momboisse F, Houy S, Chasserot-Golaz S, Heintz D, Calco V, Haeberlé AM, Espinoza FA, Sims PJ, Bailly Y, Bader MF, Gasman S (2013) Phospholipid scramblase-1-induced lipid reorganization regulates compensatory endocytosis in neuroendocrine cells. J. Neurosci. 33:3545-56.

Ceridono M, Ory S, Momboisse F, Chasserot-Golaz S, Houy S, Calco V, Haeberlé AM, Demais V, Bailly Y, Bader MF, Gasman S (2011) Selective recapture of secretory granule components after full collapse exocytosis in neuroendocrine chromaffin cells. Traffic 12(1):72-88.


The French National Centre for Scientific Research