Transcriptional and epigenetic signatures of opiates
Opiates such as morphine and heroin have been both a benediction and a curse to mankind for centuries. While they remain the most efficient and prescribed painkillers in modern medicine, their chronic use also leads in some individuals to the emergence of addiction. Once hooked, affected individuals face a lifetime struggle to control, and hopefully abstain from, opiate consumption.
A major challenge is to understand how opiates can have such devastating and protracted effects on human behaviour. Our current projects seek to answer this important clinical and societal question, by exploring the hypothesis that epigenetic mechanisms may be critically implicated in these processes. Epigenetic mechanisms, such as DNA methylation and histone marks, correspond to chemical modifications that do not modifiy the DNA sequence, but control structural and functional organization of the genome. While they have been primarily implicated in cellular differentiation, accumulating data indicates that they remain active throughout life, even in post-mitotic neurons of the adult brain, where epigenetic reprogramming is progressively associated with a series of psychiatric phenotypes, including depression, addiction, and others. In this context, our animal research program proposes to focus on the role of DNA methylation, a major epigenetic mark, in opiate effects.
To do so, we combine genetic tools that we develop in the mouse, with next-generation sequencing methodologies and behavioural testing. Our goal is to produce comprehensive characterizations, in the entire genome, of DNA methylation, histone and gene expression adaptations that are triggered by opiates. Furthermore, we seek to characterize how these molecular substrates contribute to behavioural effects of opiates, focusing on their “dark side”: the potent aversive aspects of drug withdrawal, and the emergence of emotional dysfunction during prolonged abstinence from the drug.
Importantly, we are developing experimental strategies to conduct cell-type specific analyses of the neuronal population that expresses the mu opioid receptor, and represents the primary target of opiates. This endeavour is driven by the recognition that the mammalian brain is composed of multiple intermingled cell-types that exhibit huge transcriptomic and epigenetic diversity. This complexity severely hampers the detection of subtle cell-type specific processes that account for psychiatric symptoms, and go undetected otherwise. Implementing cell-type specific strategies is therefore critical, and our strategy has the potential to define the epigenetic blueprint of opiates with unprecedented resolution. In the long-term, we hope that this work will represent a main step forward for the understanding of opiate powerful properties.
Lutz P-E, Gross JA, Dhir SK, Maussion G, Yang J, Bramoulle A, Meaney MJ, Turecki G
Biological Psychiatry 84(10):751-761.
Focusing on the opioid system for addiction biomarker discovery (review).
Belzeaux R, Lalanne T, Kieffer BL, Lutz P-E
Trends Molecular Medicine 24(2):206-220
Association of a History of Child Abuse With Impaired Myelination in the Anterior Cingulate Cortex: Convergent Epigenetic, Transcriptional, and Morphological Evidence.
Lutz P-E, Tanti A, Gasecka A, Barnett-Burns S, Kim JJ, Zhou Y, Chen GG, Wakid M, Shaw M, Almeida D, Chay M-A, Yang J, Larivière V, M’Boutchou M-N, van Kempen L, Yerko V, Prud’homme J, Davoli MA, Vaillancourt K, Théroux J-F, Bramoullé A, Zhang TY, Meaney MJ, Ernst C, Côté D, Mechawar N, Turecki G
American Journal of Psychiatry 174(12):1185-1194
Neuropathology of suicide: recent findings and future directions (Review).
Lutz P-E, Mechawar N, Turecki G
Molecular Psychiatry 22(10):1395-1412
Distinct mu, delta and kappa opioid receptor mechanisms underlie low sociability and depressive-like behaviors during heroin abstinence.
Lutz P-E, Ayranci G, Chu-Sin-Chung P, Matifas A, Koebel P, Filliol D, Befort K, Ouagazzal AM, Kieffer BL
Kappa opioid receptor antagonism and chronic antidepressant treatment have beneficial activities on social interactions and grooming deficits during heroin abstinence.
Lalanne L, Ayranci G, Befort K, Filliol D, Kieffer BL, Lutz P-E
Addiction Biology 22(4):1010-1021
Opioid receptors: distinct roles in mood disorders (Review).
Lutz P-E, Kieffer BL
Trends in Neurosciences 36(3):195-206
- Genetic engineering in the mouse (transgenic, knockout, knockin mouse lines)
- Next-generation sequencing (RNA-Seq)
- Epigenetics (WGBS, ChIP-Seq)
- Quantitative PCR, immunohistochemistry
- Behavioural models: opiate precipitated withdrawal-induced conditioned place aversion; opiate spontaneous withdrawal / abstinence
- Behavioural testing: social interactions, forced swim, open-field, novelty-suppressed feeding
- Karine Merienne & Anne-Laurence Boutillier (Laboratoire de Neurosciences Cognitives et Adaptatives, CNRS UMR 7364, University of Strasbourg, France)
- Brigitte Kieffer (Douglas Hospital Research Centre, McGill University, Montréal, Canada)
- Gustavo Turecki (McGill Group for Suicide Studies, Douglas Hospital Research Centre, McGill University, Montréal, Canada)
- Benoit Labonté (CERVO Institute, Laval University, Québec, Canada)
- Raoul Belzeaux, El Chérif Ibrahim, Eduardo Gascon (Institut de Neurosciences de la Timone, Marseille, France)
- Andrée Delahaye-Duriez
- Bruno Etain & Cynthia Marie-Claire (Paris Descartes University, INSERM UMR 1144)